Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes. / Christensen, Pernille M.; Bosteen, Markus H.; Hajny, Stefan; Nielsen, Lars B.; Christoffersen, Christina.

In: Scientific Reports, Vol. 7, 14983, 08.11.2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christensen, PM, Bosteen, MH, Hajny, S, Nielsen, LB & Christoffersen, C 2017, 'Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes', Scientific Reports, vol. 7, 14983. https://doi.org/10.1038/s41598-017-15043-y

APA

Christensen, P. M., Bosteen, M. H., Hajny, S., Nielsen, L. B., & Christoffersen, C. (2017). Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes. Scientific Reports, 7, [14983]. https://doi.org/10.1038/s41598-017-15043-y

Vancouver

Christensen PM, Bosteen MH, Hajny S, Nielsen LB, Christoffersen C. Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes. Scientific Reports. 2017 Nov 8;7. 14983. https://doi.org/10.1038/s41598-017-15043-y

Author

Christensen, Pernille M. ; Bosteen, Markus H. ; Hajny, Stefan ; Nielsen, Lars B. ; Christoffersen, Christina. / Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex

@article{00be677a01414059a58fa3c5f92fd3d9,
title = "Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes",
abstract = "Sphingosine-1-phosphate (S1P) is a bioactive lipid implicated in e.g. angiogenesis, lymphocyte trafficking, and endothelial barrier function. Erythrocytes are a main source of plasma S1P together with platelets and endothelial cells. Apolipoprotein M (apoM) in HDL carries 70% of plasma S1P, whereas 30% is carried by albumin. The current aim was to investigate the role of apoM in export of S1P from human erythrocytes. Erythrocytes exported S1P more efficiently to HDL than to albumin, particularly when apoM was present in HDL. In contrast, export of sphingosine to HDL was unaffected by the presence of apoM. The specific ability of apoM to promote export of S1P was independent of apoM being bound in HDL particles. Treatment with MK-571, an inhibitor of the ABCC1 transporter, effectively reduced export of S1P from human erythrocytes to apoM, whereas the export was unaffected by inhibitors of ABCB1 or ATPase. Thus, ABCC1 could be involved in export of S1P from erythrocytes to apoM.",
author = "Christensen, {Pernille M.} and Bosteen, {Markus H.} and Stefan Hajny and Nielsen, {Lars B.} and Christina Christoffersen",
year = "2017",
month = nov,
day = "8",
doi = "10.1038/s41598-017-15043-y",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes

AU - Christensen, Pernille M.

AU - Bosteen, Markus H.

AU - Hajny, Stefan

AU - Nielsen, Lars B.

AU - Christoffersen, Christina

PY - 2017/11/8

Y1 - 2017/11/8

N2 - Sphingosine-1-phosphate (S1P) is a bioactive lipid implicated in e.g. angiogenesis, lymphocyte trafficking, and endothelial barrier function. Erythrocytes are a main source of plasma S1P together with platelets and endothelial cells. Apolipoprotein M (apoM) in HDL carries 70% of plasma S1P, whereas 30% is carried by albumin. The current aim was to investigate the role of apoM in export of S1P from human erythrocytes. Erythrocytes exported S1P more efficiently to HDL than to albumin, particularly when apoM was present in HDL. In contrast, export of sphingosine to HDL was unaffected by the presence of apoM. The specific ability of apoM to promote export of S1P was independent of apoM being bound in HDL particles. Treatment with MK-571, an inhibitor of the ABCC1 transporter, effectively reduced export of S1P from human erythrocytes to apoM, whereas the export was unaffected by inhibitors of ABCB1 or ATPase. Thus, ABCC1 could be involved in export of S1P from erythrocytes to apoM.

AB - Sphingosine-1-phosphate (S1P) is a bioactive lipid implicated in e.g. angiogenesis, lymphocyte trafficking, and endothelial barrier function. Erythrocytes are a main source of plasma S1P together with platelets and endothelial cells. Apolipoprotein M (apoM) in HDL carries 70% of plasma S1P, whereas 30% is carried by albumin. The current aim was to investigate the role of apoM in export of S1P from human erythrocytes. Erythrocytes exported S1P more efficiently to HDL than to albumin, particularly when apoM was present in HDL. In contrast, export of sphingosine to HDL was unaffected by the presence of apoM. The specific ability of apoM to promote export of S1P was independent of apoM being bound in HDL particles. Treatment with MK-571, an inhibitor of the ABCC1 transporter, effectively reduced export of S1P from human erythrocytes to apoM, whereas the export was unaffected by inhibitors of ABCB1 or ATPase. Thus, ABCC1 could be involved in export of S1P from erythrocytes to apoM.

U2 - 10.1038/s41598-017-15043-y

DO - 10.1038/s41598-017-15043-y

M3 - Journal article

C2 - 29118354

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 14983

ER -

ID: 188526861