An in vitro larval migration assay for assessing anthelmintic activity of different drug classes against Ascaris suum
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An in vitro larval migration assay for assessing anthelmintic activity of different drug classes against Ascaris suum. / Zhao, Jianguo; Williams, Andrew R; Hansen, Tina Vicky Alstrup; Thamsborg, Stig M; Cai, Jianping; Song, Shuaibao; Chen, Gang; Kang, Ming; Zhang, Zhuangzhi; Liu, Sheng-Qun; Han, Qian.
In: Veterinary Parasitology, Vol. 238, 30.04.2017, p. 43-48.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - An in vitro larval migration assay for assessing anthelmintic activity of different drug classes against Ascaris suum
AU - Zhao, Jianguo
AU - Williams, Andrew R
AU - Hansen, Tina Vicky Alstrup
AU - Thamsborg, Stig M
AU - Cai, Jianping
AU - Song, Shuaibao
AU - Chen, Gang
AU - Kang, Ming
AU - Zhang, Zhuangzhi
AU - Liu, Sheng-Qun
AU - Han, Qian
N1 - Copyright © 2017 Elsevier B.V. All rights reserved.
PY - 2017/4/30
Y1 - 2017/4/30
N2 - In vitro methods have been developed for the detection of anthelmintic resistance in a range of nematode species. However, the life cycle of Ascaris suum renders the commonly used egg hatch assay and larval development assay unusable. In this study we developed a combined multi-well culture and agar gel larval migration assay to test the effect of benzimidazole and tetrahydropyrimidin/imidazothiazole anthelmintics against nine isolates of A. suum collected from locations in China and Denmark. Drugs tested were thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel. The percentages of larvae that migrated to the surface of each treated and control well were used to calculate the drug concentration which inhibits 50% of the larvae migration (EC50). The values of EC50 of thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel against A. suum isolates ranged 74-150, 4.9-13.9, 2.3-4.3, 358-1150 and 1100-4000nM, respectively. This combined multi-well culture and agar gel larval migration assay was a sensitive bioassay for anthelmintic activity and could serve as an in vitro method to detect for lowered drug efficacy against A. suum or possibly to screen for anthelmintic drug candidates.
AB - In vitro methods have been developed for the detection of anthelmintic resistance in a range of nematode species. However, the life cycle of Ascaris suum renders the commonly used egg hatch assay and larval development assay unusable. In this study we developed a combined multi-well culture and agar gel larval migration assay to test the effect of benzimidazole and tetrahydropyrimidin/imidazothiazole anthelmintics against nine isolates of A. suum collected from locations in China and Denmark. Drugs tested were thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel. The percentages of larvae that migrated to the surface of each treated and control well were used to calculate the drug concentration which inhibits 50% of the larvae migration (EC50). The values of EC50 of thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel against A. suum isolates ranged 74-150, 4.9-13.9, 2.3-4.3, 358-1150 and 1100-4000nM, respectively. This combined multi-well culture and agar gel larval migration assay was a sensitive bioassay for anthelmintic activity and could serve as an in vitro method to detect for lowered drug efficacy against A. suum or possibly to screen for anthelmintic drug candidates.
U2 - 10.1016/j.vetpar.2017.03.014
DO - 10.1016/j.vetpar.2017.03.014
M3 - Journal article
C2 - 28342672
VL - 238
SP - 43
EP - 48
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
ER -
ID: 178262600