Adhesion, growth, and matrix production by fibroblasts on laminin substrates.

Research output: Contribution to journalJournal articleResearchpeer-review

J R Couchman, M Höök, D A Rees, R Timpl

Human embryonic skin fibroblasts have been shown to attach and spread on laminin substrates in the absence of protein synthesis and presence of fibronectin-depleted serum and anti-fibronectin antibodies. Rates of attachment and the type of spreading are virtually identical on fibronectin and laminin-coated substrates with the development of microfilament bundles and focal adhesions. Antibodies to laminin, but not fibronectin, will prevent or reverse fibroblast adhesion to laminin, whereas antibodies to fibronectin but not laminin will give similar results on fibronectin-coated substrates. These and other results indicate that fibroblasts possess distinct receptors for laminin and fibronectin which on contact with suitable substrates promote adhesion through interaction with common intermediates. This type of adhesion is compatible with subsequent growth and extracellular matrix production.
Original languageEnglish
JournalJournal of Cell Biology
Volume96
Issue number1
Pages (from-to)177-83
Number of pages6
ISSN0021-9525
Publication statusPublished - 1983

Bibliographical note

Keywords: Antibodies; Cell Adhesion; Cell Division; Cell Movement; Cells, Cultured; Cytoskeleton; Fibroblasts; Fibronectins; Glycoproteins; Humans; Laminin; Procollagen; Skin

ID: 5167837