A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response

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Melanie Blasius, Sebastian A Wagner, Chuna Ram Choudhary, Jiri Bartek, Stephen P Jackson

RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.

Original languageEnglish
JournalGenes & Development
Volume28
Issue number18
Pages (from-to)1977-1982
Number of pages6
ISSN0890-9369
DOIs
Publication statusPublished - 4 Sep 2014

ID: 123734095