MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND: The brain is a major site of microRNA (miRNA) gene expression, but the spatial expression patterns of miRNAs within the brain have not yet been fully covered. METHODOLOGY/PRINCIPAL FINDINGS: We have characterized the regional expression profiles of miRNAs in five distinct regions of the adult rat brain: amygdala, cerebellum, hippocampus, hypothalamus and substantia nigra. Microarray profiling uncovered 48 miRNAs displaying more than three-fold enrichment between two or more brain regions. Notably, we found reciprocal expression profiles for a subset of the miRNAs predominantly found (> ten times) in either the cerebellum (miR-206 and miR-497) or the forebrain regions (miR-132, miR-212, miR-221 and miR-222). CONCLUSIONS/SIGNIFICANCE: The results indicate that some miRNAs could be important for area-specific functions in the brain. Our data, combined with previous studies in mice, provides additional guidance for future investigations of miRNA functions in the brain.
Original language | English |
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Journal | PLoS ONE |
Volume | 4 |
Issue number | 10 |
Pages (from-to) | e7225 |
Number of pages | 7 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2009 |
Bibliographical note
Keywords: Animals; Cerebellum; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation; Male; Mice; MicroRNAs; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Prosencephalon; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, RNA
ID: 20010895